Barth - who co-starred in MacFarlane's Ted movies - told him that Weinstein had made repeated and unwanted moves on her.
The morning of the Academy Award nominations, MacFarlane and Emma Stone announced the nominees for Best Supporting Actress - which that year were Sally Field, Anne Hathaway, Jacki Weaver, Helen Hunt and Amy Adams.
Barth, like others in the article, alleges that she was invited under the guise of a business meeting to Weinstein's hotel room, where she says he proceeded to demand a naked massage.
"Family Guy" creator Seth Macfarlane has finally broken his silence about his 2013 Oscar dig at Harvey Weinstein, saying it "came from a place of loathing and anger" because movie mogul allegedly made advances towards his friend Jessica Berth.
"To the people slamming Seth for not "doing" anything, please STOP!", she wrote on Twitter.
"I couldn't resist the opportunity to take a hard swing in his direction".
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"Make no mistake, this came from a place of loathing and anger", MacFarlane continued. Barth tweeted. "He stood by me and respected my wishes that he not retaliate in any way". McGowan claimed that Affleck knew years ago because she told him about Weinstein and said Affleck told her, "Goddamnit!"
Mounting numbers of women are coming forward with allegations of sexual assault and misconduct against Mr Weinstein, most recently model Cara Delevingne. Then on Tuesday, the list grew even longer, when Gwyneth Paltrow and Angelina Jolie accused Weinstein of sexually harassing them earlier in their respective careers. "Mr. Weinstein has further confirmed that there were never any acts of retaliation against any women for refusing his advances".
MacFarlane wasn't the only comedian to reference Weinstein's alleged sexual misdeeds long before the New York Times story made them public; Tina Fey's hit NBC sitcom 30 Rock took aim at Weinstein on two occasions, with Jane Krakowski's character Jenna Maroney noting, "I have turned down sex with Harvey Weinstein on no less than three occasions".
Weinstein has separately issued an apology and said he is going to get therapy.